













NEW HPLC Column Chromegabond HC-C18 150x4.6mm 5µm compare Kromasil C18
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Column: Chromegabond HC-C18 150 x 4.6 mm 5µm
Catalog No.: 135221-HC-C18
pore size = 100° A;
surface area = 350 g2/m; Carbon = 22%;
pH range = 2-8
Analytics:
* Aromatic Acids:
Eluent: 20% Acetonitrile/80% Water 01.% TFA
Flow rate: 1 mL/min.
Detection: UV @ 254 nm
1. Phenylacetic acid
2. Benzoic acid
3. o-toluic acid
4. p-toluic acid
* Substituted Anilines:
Eluent: 48% Methanol/52% 10 nM sodium phosphate pH = 2.5
Flow rate: 1 mL/min.
Detection: UV @ 254 nm
1. p-anisidine
2. m-toluidine
3. 3-aminobenzonitrile
4. 4-chloroaniline
5. 3-chloroaniline
6. 2-chloroaniline
* Acids and Bases:
Eluent: 50% Methanol/50% Buffer 100 nM
H3PO4 pH = 2.4
Flow rate: 1 mL/min.
Detection: UV @ 254 nm
1. p-Amino benzoic acid
2. o-Amino benzoic acid
3. p-Hydroxybenzoic acid
4. Benzyl Alcohol
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